• 产品编号:HIV-001
    • 产品名称:重组HIV-1蛋白酶/HIV-1 Protease
    • 产品规格:2μg/10μg/10μg
    • 价  格:700.00/1800.00/
    • 供 应 商:ProSpecbio
    • 库  存:
  • Catalogue Number HIV-001
    Introduction HIV-1 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.
    Description

    HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).

    Source Escherichia Coli.
    Physical Appearance Sterile filtered colorless clear solution.
    Formulation The HIV-1 Protease solution (0.25mg/1ml) is formulated in 20mM acetate, 200mM NaCl, 1mM EDTA, 0.5mM DTT, pH5.0 and 10% glycerol.
    Storage Buffer 20mM HEPES, pH7.0 and 10% Glycerol.
    Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    Please avoid freeze thaw cycles.
    Kinetic parameters Km=15.1μM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF(NO2)VRKA (F(NO2) ... p-nitrophenylalanine).
    Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.